Part:BBa_K2915100:Design

AFP-Iu1 6-His-Tag with TEV site

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We add a 6Hig-Tag onto the "antifreeze protein" sequence which allows us to purify our proteins. We optimized the codons to be able to produce these proteins into the K12 E.coli strain.

Source

nucleics acids